• Qual­i­ta­tive and Quan­ti­ta­tive Research of Genet­i­cally Modi­fied Organ­isms (GMO)
    Qual­i­ta­tive analysis for GMO screening to detect the pres­ence of genet­i­cally modi­fied organ­isms in food­stuffs, animal feed and plants (soy, corn, rice, colza, cotton, beets, apples, beans, lupin, papaya, pota­toes, wheat). Quan­ti­ta­tive analysis to research trans­genic events of soy and corn.
  • Genetic analysis of the muta­tion of the cattle myostatin gene to distin­guish the Pied­mont breed from the other breeds
    Myostatin is a protein that controls the devel­op­ment of muscle mass in the foetus and while growing. In the cattle breeds that present the double-muscled phenom­enon, there is a specific muta­tion that renders this protein weak making it so the muscles grown more than usual. The genetic muta­tion of the Pied­mont breed is different to that of the other double-muscled breeds and this makes it possible to distin­guish it through DNA analysis. The Laem­megroup Mole­c­ular Biology Labo­ra­tory is able to carry out analyses in Real Time PCR of the G>A muta­tion in posi­tion 938 of the myostatin gene on a DNA sample extracted from bovine meat.
  • Genetic analysis of bovine beta casein gene muta­tion to discern condi­tions of homozy­gosis or heterozigosis from a single carrier
    About 25% of milk proteins are consti­tuted by whey protein and non-protein nitroge­nous constituents, while the remainder is casein, the protein involved in the produc­tion of cheese. Beta casein repre­sents approx­i­mately 36% of the overall amount of casein. Of the 13 vari­ants of beta casein, the most common are A1 and A2, whose alleles are co-domi­nant. On a genetic level, the differ­ence between the two vari­ants is based on the differ­ence of a single nucleotide, which is equiv­a­lent to the C>A muta­tion in posi­tion 473 of the bovine beta casein gene. The Laem­megroup Micro­bi­ology Labo­ra­tory is able to carry out analyses in Real Time PCR on the C>A muta­tion, thus discerning the bovine on the basis of the mutant A1A1 homozy­gous, A1A2 heterozy­gous and non-mutant A2A2 homozy­gous genotypes.
  • Iden­ti­fi­ca­tion of animal origin DNA in food­stuffs and cheese. With regards preven­tive measures adopted in Italy for Bovine Spongi­form Encephalopathy (BSE) with partic­ular atten­tion to the animal feed industry, since 1994 the Ministry has prohib­ited admin­is­tering proteins deriving from mammalian tissues in the diet of grazers. To monitor this prohi­bi­tion, a control system was put in place by the compe­tent author­i­ties to inter­vene in every stage of the supply chain.
  • Iden­ti­fi­ca­tion of path­o­genic micro-organisms
    • Listeria mono­cy­to­genes
    • Salmo­nella spp.
    • Shiga Toxin producing Escherichia Coli (STEC)
  • Qual­i­ta­tive deter­miners through Real Time PCR of some aller­gens like soy, celery and pistachio
    Other aller­gens are researched in the Chem­ical Labo­ra­tory through the ELISA method.

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